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1. (WO2017201102) METHOD OF IMPROVED SEQUENCING BY STRAND IDENTIFICATION

Pub. No.:    WO/2017/201102    International Application No.:    PCT/US2017/032980
Publication Date: Fri Nov 24 00:59:59 CET 2017 International Filing Date: Wed May 17 01:59:59 CEST 2017
IPC: C12N 15/10
C12P 19/34
C12Q 1/68
C40B 50/06
Applicants: ACCURAGEN HOLDINGS LIMITED
WENG, Li
Inventors: WENG, Li
FAHAM, Malek
LIN, Shengrong
TANG, Ling, Fung
LU, Yontao
SUN, Zhaohui
WANG, Yingyu
Title: METHOD OF IMPROVED SEQUENCING BY STRAND IDENTIFICATION
Abstract:
In some aspects, the present disclosure provides methods for identifying sequence variants, as well as methods of determining copy number of a genetic locus in a sample. Systems and kits for performing methods of the disclosure, as well as compositions produced by or useful in methods of the disclosure are also provided. In some embodiments, methods comprise extending 3' ends of polynucleotides by adding one or more pre-determined nucleotides. In some embodiments, methods comprise use of a strand-tagging sequence.