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|1. (WO2017201102) METHOD OF IMPROVED SEQUENCING BY STRAND IDENTIFICATION|
|Applicants:||ACCURAGEN HOLDINGS LIMITED
TANG, Ling, Fung
|Title:||METHOD OF IMPROVED SEQUENCING BY STRAND IDENTIFICATION|
In some aspects, the present disclosure provides methods for identifying sequence variants, as well as methods of determining copy number of a genetic locus in a sample. Systems and kits for performing methods of the disclosure, as well as compositions produced by or useful in methods of the disclosure are also provided. In some embodiments, methods comprise extending 3' ends of polynucleotides by adding one or more pre-determined nucleotides. In some embodiments, methods comprise use of a strand-tagging sequence.