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1. (WO2017200486) HUMAN STEM CELL DERIVED ENDOTHELIAL CELLS, ENDOTHELIAL- HEPATOCYTE CO-CULTURE SYSTEM AND USES THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/200486    International Application No.:    PCT/SG2017/050255
Publication Date: 23.11.2017 International Filing Date: 16.05.2017
Chapter 2 Demand Filed:    19.03.2018    
IPC:
C12N 5/071 (2010.01), G01N 33/50 (2006.01)
Applicants: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG)
Inventors: CHEUNG, Christine; (SG).
NARMADA, Balakrishnan Chakrapani; (SG).
GOH, Yeek Teck; (SG)
Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531 Robinson Road Post Office Singapore 903031 (SG)
Priority Data:
10201603939U 17.05.2016 SG
Title (EN) HUMAN STEM CELL DERIVED ENDOTHELIAL CELLS, ENDOTHELIAL- HEPATOCYTE CO-CULTURE SYSTEM AND USES THEREOF
(FR) CELLULES ENDOTHÉLIALES DÉRIVÉES DE CELLULES SOUCHES HUMAINES, SYSTÈME DE CO-CULTURE D'HÉPATOCYTES ET DE CELLULES ENDOTHÉLIALES ET LEURS UTILISATIONS
Abstract: front page image
(EN)The present disclosure provides a method of deriving endothelial cells, comprising (a) culturing lateral plate mesoderm cells under oxygen-deprived condition to obtain endothelial lineage cells; and (b) culturing endothelial cells from (a) on an extracellular matrix to maintain and expand the endothelial lineage cells. Also disclosed herein is a cell co-culture system comprising an endothelial cell culture and a hepatocyte cell culture, as well as a microfluidic-based system comprising said cell co-culture system. Also disclosed herein is a method of disease modelling or drug testing using said cell co-culture system or said microfluidic-based system.
(FR)La présente invention concerne un procédé de dérivation de cellules endothéliales, comprenant (a) la culture de cellules mésodermiques de plaque latérale dans des conditions dépourvues d'oxygène pour obtenir des cellules de lignée endothéliale ; et (b) la culture de cellules endothéliales à partir de (a) sur une matrice extracellulaire afin de maintenir et d'étendre les cellules de lignée endothéliale. L'invention concerne également un système de co-culture cellulaire comprenant une culture de cellules endothéliales et une culture de cellules hépatiques, ainsi qu'un système microfluidique comprenant ledit système de co-culture cellulaire. L'invention concerne également un procédé de modélisation de maladie ou d’essai de médicament mettant en œuvre ledit système de co-culture cellulaire ou ledit système à base microfluidique.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)