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1. (WO2017198890) MYCELIOPHTHORA THERMOPHILA HOST CELL HAVING IMPROVED CELLULOLYTIC ACTIVITY AND ENZYMATIC COMPOUNDS PRODUCED WITH SAME

Pub. No.:    WO/2017/198890    International Application No.:    PCT/ES2017/070339
Publication Date: Fri Nov 24 00:59:59 CET 2017 International Filing Date: Tue May 23 01:59:59 CEST 2017
IPC: C12N 1/15
C12P 19/14
C12P 7/10
C12R 1/645
Applicants: ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S.A.
Inventors: DÍEZ GARCÍA, Bruno
VALBUENA CRESPO, Noelia
REYES SOSA, Francisco Manuel
MORENO PÉREZ, Antonio Javier
PÉREZ GÓMEZ, Dolores
PLATERO GÓMEZ, Ana Isabel
MARTÍN PÉREZ, Lucía
GAVALDÁ MARTÍN, Sandra
VIÑAS DE LA CRUZ, Laura
SÁNCHEZ ZAMORANO, Laura
ÁLVAREZ NÚÑEZ, Consolación
BERMÚDEZ ALCÁNTARA, María De Los Ángeles
ROCHA MARTÍN, Javier
LEDESMA GARCÍA, Laura
ARJONA ANTOLÍN, Ricardo
RAMOS MARTÍN, Juan Luis
Title: MYCELIOPHTHORA THERMOPHILA HOST CELL HAVING IMPROVED CELLULOLYTIC ACTIVITY AND ENZYMATIC COMPOUNDS PRODUCED WITH SAME
Abstract:
The invention relates to a host cell, preferably a Myceliophthora thermophila cell, which presents a lower expression and/or secretion of non-contributory cellulolytic enzymes, preferably wherein the non-contributory cellulolytic enzyme is endoglucanase 6 comprising SEQ ID NO: 2, thereby promoting the presence of contributory cellulolytic enzymes in the enzymatic cocktail synthesised by said host cell. The invention also relates to the use of said host cells and the enzymatic cocktails synthesised by said host cells for the production of fermentable sugars of biomass, and a method for producing bioproducts, preferably bioethanol, comprising the use of said host cell or the composition according to the invention.