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1. (WO2017198562) METHOD OF PRODUCING DOMAINS OF PROTEIN
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Pub. No.: WO/2017/198562 International Application No.: PCT/EP2017/061455
Publication Date: 23.11.2017 International Filing Date: 12.05.2017
IPC:
C12N 15/85 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
Applicants:
SIEMENS HEALTHCARE GMBH [DE/DE]; Henkestr. 127 91052 Erlangen, DE
Inventors:
ALTHAUS, Harald; DE
GALGALKAR, Sudipa; IN
GURPUR, Praveen Bhat; IN
HAHN, Martin; DE
KAPPEL, Andreas; DE
SCHWARZ, Herbert; DE
Agent:
BALS & VOGEL PATENTANWÄLTE; Königinstraße 11 RGB 80539 München, DE
Priority Data:
20163101723219.05.2016IN
Title (EN) METHOD OF PRODUCING DOMAINS OF PROTEIN
(FR) PROCÉDÉ DE PRODUCTION DE DOMAINES DE PROTÉINES
Abstract:
(EN) The present invention relates to a method (10) for producing one or more domains of protein in their native state. The method comprises the steps of introducing one or more enzyme recognition sites (3) within a gene for a recombinant protein (2), wherein said gene for recombinant protein (2) encodes for said one or more domains of protein; forming an expression construct (4) by cloning said gene for said recombinant protein (2) into an expression vector (1); transferring said expression construct (4) into mammalian cells (5); expressing said gene for recombinant protein (2) containing one or more enzyme recognition sites (3) in said mammalian cells (5); contacting said expressed recombinant protein with an enzyme specific to said one or more enzyme recognition sites; and producing one or more domains of proteins in their native state.
(FR) La présente invention concerne un procédé (10) de production d'au moins un domaine de protéine dans son état natif. Le procédé comprend les étapes consistant à : introduire au moins un site de reconnaissance enzymatique (3) dans un gène d'une protéine recombinante (2), lequel gène de la protéine recombinante (2) code pour ledit au moins un domaine de protéine ; former une construction d'expression (4) par clonage dudit gène de ladite protéine recombinante (2) dans un vecteur d'expression (1) ; transférer ladite construction d'expression (4) dans des cellules mammifères (5) ; exprimer ledit gène de la protéine recombinante (2) contenant au moins un site de reconnaissance enzymatique (3) dans lesdites cellules mammifères (5) ; mettre en contact ladite protéine recombinante exprimée avec une enzyme spécifique de l'au moins un site de reconnaissance enzymatique ; et produire au moins un domaine de protéine dans son état natif.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)