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1. (WO2017198488) IMPROVED-PERFORMANCE PROTEASES
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Pub. No.: WO/2017/198488 International Application No.: PCT/EP2017/060947
Publication Date: 23.11.2017 International Filing Date: 08.05.2017
IPC:
C12N 9/54 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
54
bacteria being Bacillus
Applicants:
HENKEL AG & CO. KGAA [DE/DE]; Henkelstr. 67 40589 Düsseldorf, DE
Inventors:
MUSSMANN, Nina; DE
O'CONNELL, Timothy; DE
HERBST, Daniela; DE
PRÜSER, Inken; DE
DEGERING, Christian; DE
STRAUSS, Daria; DE
EGGERT, Thorsten; DE
LEGGEWIE, Christian; DE
Priority Data:
10 2016 208 463.118.05.2016DE
Title (EN) IMPROVED-PERFORMANCE PROTEASES
(FR) PROTÉASES À PERFORMANCE AMÉLIORÉE
(DE) LEISTUNGSVERBESSERTE PROTEASEN
Abstract:
(EN) The invention relates to proteases having an amino acid sequence with at least 70% sequence identity to the amino acid sequence given in SEQ ID No. 1, across its whole length, and comprising an amino acid substitution on at least one of the positions P9, K22, V28, A29, A48, Q62, T78, L82, V84, S87, D101, N130, G131, T133, N144, G166, T215, S216, S224, N240, N252, S260 or Q271, relating in each case to the numbering according to SEQ ID No. 1. The invention also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.
(FR) L'invention concerne des protéases comprenant une séquence d'acides aminés présentant sur sa longueur totale au moins 70 % d’identité de séquence avec la séquence d'acides aminés indiquée dans SEQ ID NO:1 et présentant une substitution d'acide aminé à au moins une des positions correspondant aux positions P9, K22, V28, A29, A48, Q62, T78, L82, V84, S87, D101, N130, G131, T133, N144, G166, T215, S216, S224, N240, N252, S260 ou Q271 respectivement par rapport à la numérotation selon SEQ ID NO:1, ainsi que leur production et leur utilisation. De telles protéases offrent une très bonne performance de nettoyage.
(DE) Die Erfindung betrifft Proteasen umfassend eine Aminosäuresequenz, die mindestens 70 % Sequenzidentität mit der in SEQ ID NO:1 angegebenen Aminosäuresequenz über deren Gesamtlänge aufweist und die eine Aminosäuresubstitution an mindestens einer der Positionen, die den Positionen P9, K22, V28, A29, A48, Q62, T78, L82, V84, S87, D101, N130, G131, T133, N144, G166, T215, S216, S224, N240, N252, S260 oder Q271, jeweils bezogen auf die Nummerierung gemäß SEQ ID NO:1, entsprechen, aufweisen sowie deren Herstellung und Verwendung. Derartige Proteasen zeigen eine sehr gute Reinigungsleistung.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)