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1. (WO2017197546) Β-MANNANASE MRMMAN5A AND ENCODING GENE AND APPLICATION THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/197546 International Application No.: PCT/CN2016/000546
Publication Date: 23.11.2017 International Filing Date: 28.09.2016
IPC:
C12N 9/42 (2006.01) ,C12N 15/56 (2006.01) ,C12N 15/11 (2006.01) ,C12P 19/14 (2006.01)
Applicants: CHINA AGRICULTURAL UNIVERSITY[CN/CN]; No.17, Tsinghua East Road, Haidian District Beijing 100083, CN
Inventors: JIANG, Zhengqiang; CN
LI, Yanxiao; CN
YAN, Qiaojuan; CN
YI, Ping; CN
LIU, Xueqiang; CN
Agent: BEIJING WEIPINGZHIYE PATENT AGENT FIRM; CN
Priority Data:
201610324502.917.05.2016CN
Title (EN) Β-MANNANASE MRMMAN5A AND ENCODING GENE AND APPLICATION THEREOF
(FR) Β-MANNANASE MRMMAN5A ET GÈNE CODANT ET SON APPLICATION
(ZH) 一种β-甘露聚糖酶mRmMan5A及其编码基因与应用
Abstract:
(EN) Provided are a β-mannanase mRmMan5A and an encoding gene and an application thereof The present β-mannanase mRmMan5A has the following advantages compared to original β-mannanase mRmMan5A: optimal pH 4.5 and optimal temperature 65° C. Introducing the present protein encoding gene into pichia pastoris achieves highly effective expression. The present β-mannanase mRmMan5A is used in palm meal having undergone hydrolytic steam explosion processing, the hydrolysate mainly comprising a mannan oligosaccharide having a polymerisation degree of 2-4, the mannan oligosaccharide yield being 19.6%, and the mannan conversion rate being about 60%.
(FR) La présente invention décrit une β-mannanase mRmMan5A et un gène codant et son application. La présente β-mannanase mRmMan5A offre les avantages suivants par rapport à la β-mannanase mRmMan5A d’origine : pH 4,5 optimal et température optimale de 65 °C. L’introduction du présent gène codant pour la protéine dans Pichia pastoris permet d’atteindre une expression hautement efficace. La présente β-mannanase mRmMan5A est utilisée dans la farine de palme ayant subi un traitement par explosion hydrolytique à la vapeur, l’hydrolysat comprenant principalement un oligosaccharide mannane présentant un degré de polymérisation de 2 à 4, le rendement de l’oligosaccharide mannane étant de 19,6 %, et le taux de conversion du mannane étant d’environ 60 %.
(ZH) 提供了一种β-甘露聚糖酶mRmMan5A,其编码基因,以及它们的应用。所述的β-甘露聚糖酶mRmMan5A,与原始β-甘露聚糖酶RmMan5A相比具有以下优点:最适pH 4.5,最适温度65℃。将该蛋白的编码基因导入毕赤酵母中实现了高效表达。将该β-甘露聚糖酶用于水解汽爆处理后的棕榈粕,水解液中主要为聚合度为2-4之间的甘露寡糖,甘露寡糖得率为19.6%,甘露聚糖转化率约为60%。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)