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1. WO2017197098 - DIRECT SELECTION OF CELLS EXPRESSING HIGH LEVELS OF HETEROMERIC PROTEINS USING GLUTAMINE SYNTHETASE INTRAGENIC COMPLEMENTATION VECTORS

Publication Number WO/2017/197098
Publication Date 16.11.2017
International Application No. PCT/US2017/032139
International Filing Date 11.05.2017
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
65
using markers
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67
General methods for enhancing the expression
C12N 15/65 (2006.01)
C12N 15/67 (2006.01)
CPC
C07K 16/00
C07K 16/065
C07K 16/18
C07K 2319/30
C12N 15/1055
C12N 15/65
Applicants
  • AMGEN INC. [US/US]; One Amgen Center Drive Thousand Oaks, California 91320, US
Inventors
  • KETCHEM, Randal R.; US
  • MCGREW, Jeffrey T.; US
  • FOMINA YADLIN, Dina A.; US
  • MUNRO, Trent P.; US
  • AGRAWAL, Neeraj Jagdish; US
  • DARIS, Kristine M.; US
Agents
  • ANDALIS, Alex A.; US
Priority Data
62/334,96611.05.2016US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIRECT SELECTION OF CELLS EXPRESSING HIGH LEVELS OF HETEROMERIC PROTEINS USING GLUTAMINE SYNTHETASE INTRAGENIC COMPLEMENTATION VECTORS
(FR) SÉLECTION DIRECTE DE CELLULES EXPRIMANT DES TENEURS ÉLEVÉES EN PROTÉINES HÉTÉROMÈRES À L'AIDE DE VECTEURS DE COMPLÉMENTATION INTRAGÉNIQUE DE GLUTAMINE SYNTHÉTASE
Abstract
(EN)
This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.
(FR)
Cette invention concerne le domaine général de l'expression recombinée de polypeptides dans une culture de cellules animales. Plus spécifiquement, l'invention concerne une sélection améliorée de cellules transfectées par des vecteurs modifiés par recombinaison conçus pour exprimer des polypeptides, en particulier des polypeptides hétéromultimères.
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