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1. (WO2017195887) SUGAR-CHAIN CONCENTRATION COLUMN AND SUGAR-CHAIN CONCENTRATION METHOD

Pub. No.:    WO/2017/195887    International Application No.:    PCT/JP2017/018037
Publication Date: Fri Nov 17 00:59:59 CET 2017 International Filing Date: Sat May 13 01:59:59 CEST 2017
IPC: G01N 30/06
B01D 15/30
B01D 15/32
C08B 37/00
G01N 1/10
G01N 1/28
G01N 27/62
G01N 30/88
Applicants: PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
公立大学法人横浜市立大学
Inventors: OHTA, Yuki
太田 悠葵
KAWASAKI, Nana
川崎 ナナ
TAKAKURA, Daisuke
高倉 大輔
Title: SUGAR-CHAIN CONCENTRATION COLUMN AND SUGAR-CHAIN CONCENTRATION METHOD
Abstract:
Disclosed is a novel sugar-chain concentration means that is capable of handling a minute amount of a sample and increasing the concentration of a glycopeptide or a free sugar chain within a short period of time. This sugar-chain concentration column includes, in the column, a solid-phase extraction carrier layer, and a polymeric polyol layer directly or indirectly layered on the solid-phase extraction carrier layer. An octadecyl silyl silica gel or the like, and cellulose fine crystals or the like can be suitably used as the solid-phase extraction carrier and the polymeric polyol, respectively. The column can be used for increasing all concentrations of N-bound type sugar chains, O-bound type sugar chains, and free sugar chains.