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1. (WO2017191210) GENOME EDITING BY CRISPR-CAS9 IN FILAMENTOUS FUNGAL HOST CELLS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/191210 International Application No.: PCT/EP2017/060570
Publication Date: 09.11.2017 International Filing Date: 03.05.2017
IPC:
C12N 15/80 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
Applicants: NOVOZYMES A/S[DK/DK]; Krogshoejvej 36 2880 Bagsvaerd, DK
Inventors: KLITGAARD, Dorte, M.K.; DK
Priority Data:
16168301.604.05.2016EP
17168453.327.04.2017EP
Title (EN) GENOME EDITING BY CRISPR-CAS9 IN FILAMENTOUS FUNGAL HOST CELLS
(FR) ÉDITION DE GÉNOME PAR CRISPR-CAS9 DANS DES CELLULES HÔTES FONGIQUES FILAMENTEUSES
Abstract:
(EN) The present invention relates to methods for modifying at least one target sequence in the genome of a filamentous fungal host cell by employing a Class II Cas9 enzyme, e.g., the S. pyogenes Cas9, together with a suitable guide RNA for each target sequence to generate a site-specific cut or nick in at least one genome target sequence, followed by the repair of the cut(s) and/or nick(s) via integration of one or more modified modified donor part of the filamentous fungal host cell genome through double recombination on each side of the nick(s).
(FR) La présente invention concerne des méthodes de modification d'au moins une séquence cible dans le génome d’une cellule hôte fongique filamenteuse par utilisation d'une enzyme Cas9 de classe II, par ex., la Cas9 de S. pyogenes, conjointement à un ARN guide approprié pour chaque séquence cible afin de générer une coupure ou rupture spécifique du site dans au moins une séquence cible du génome, suivie de la réparation de la/des coupure(s) et/ou de la/des rupture(s) par intégration d’au moins un élément donneur modifié du génome de la cellule hôte fongique filamenteuse par double recombinaison de chaque côté de la/des rupture(s).
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)