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1. (WO2017191193) APPARATUS AND METHOD FOR LIGHT-SHEET-LIKE ILLUMINATION OF A SAMPLE

Pub. No.:    WO/2017/191193    International Application No.:    PCT/EP2017/060545
Publication Date: Fri Nov 10 00:59:59 CET 2017 International Filing Date: Thu May 04 01:59:59 CEST 2017
IPC: G02B 21/10
G02B 21/16
G02B 21/36
G02B 27/28
Applicants: LEICA MICROSYSTEMS CMS GMBH
Inventors: SCHUMANN, Christian
Title: APPARATUS AND METHOD FOR LIGHT-SHEET-LIKE ILLUMINATION OF A SAMPLE
Abstract:
An apparatus (10) for light-sheet-like illumination of a sample is described, said apparatus comprising a light source (12) for producing an illumination beam (26), and a focusing system (14) for focusing the illumination beam (26) to form a light-sheet-like illumination light distribution, by means of which a focal plane (E) of the sample can be illuminated. The apparatus (10) further comprises an imaging optical unit (18) for imaging the light-sheet-like illumination light distribution into the focal plane (E) of the sample and, arranged between the focusing system (14) and the imaging optical unit (18), a polarization element (16) for splitting the illumination beam (26) into two differently polarized partial beams (34, 36), which propagate into the imaging optical unit (18) with different directions of propagation, as a result of which the light-sheet-like illumination light distribution can be imaged by the imaging optical unit (18) in the form of two differently polarized light sheets, which are superposed on one another in the focal plane (E).