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Machine translation
1. (WO2017191007) CELL-FREE PROTEIN EXPRESSION USING ROLLING CIRCLE AMPLIFICATION PRODUCT
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/191007    International Application No.:    PCT/EP2017/059922
Publication Date: 09.11.2017 International Filing Date: 26.04.2017
IPC:
C12N 15/63 (2006.01), C12N 15/67 (2006.01)
Applicants: GENERAL ELECTRIC COMPANY [US/US]; 1 River Road Schenectady, New York 12345 (US).
GE HEALTHCARE BIO-SCIENCES AB [SE/SE]; Björkgatan 30 751 84 Uppsala (SE) (MG only)
Inventors: NELSON, John, Richard; (US).
DUTHIE, Robert, Scott; (US).
GAO, Wei; (US).
KVAM, Erik, Leeming; (US)
Agent: GE HEALTHCARE BIO-SCIENCES AB; Patent Department, Björkgatan 30 751 84 Uppsala (SE).
ALDENBÄCK, Ulla; (SE).
LARSSON, Anders; (SE).
SÖRBY, Lennart; (SE)
Priority Data:
15/145,838 04.05.2016 US
Title (EN) CELL-FREE PROTEIN EXPRESSION USING ROLLING CIRCLE AMPLIFICATION PRODUCT
(FR) EXPRESSION D'UNE PROTÉINE DANS UN SYSTÈME D'EXPRESSION ACELLULAIRE À L'AIDE D'UN PRODUIT D'AMPLIFICATION EN CERCLE ROULANT
Abstract: front page image
(EN)Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell- free expression system.
(FR)L'invention concerne des procédés de transcription et de traduction in vitro à partir d'un produit d'amplification en cercle roulant (RCA). Les procédés consistent à fournir un produit RCA double brin, le produit RCA double brin étant constitué essentiellement de répétitions en tandem d'une séquence d'expression minimale. Les procédés comprennent en outre l'expression d'une protéine à partir du produit RCA double brin dans un système d'expression acellulaire.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)