WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Options
Query Language
Stem
Sort by:
List Length
Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2017190012) METHOD OF NUCLEIC ACID SEQUENCE DETERMINATION
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/190012 International Application No.: PCT/US2017/030135
Publication Date: 02.11.2017 International Filing Date: 28.04.2017
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants: OMNIOME, INC.[US/US]; 10575 Roselle Street San Diego, California 92121, US
Inventors: VIJAYAN, Kandaswamy; US
IYIDOGAN, Pinar; US
Agent: THOMAS, Tiffany B.; US
Priority Data:
62/329,48929.04.2016US
62/444,73310.01.2017US
Title (EN) METHOD OF NUCLEIC ACID SEQUENCE DETERMINATION
(FR) PROCÉDÉ DE DÉTERMINATION D’UNE SÉQUENCE D’ACIDES NUCLÉIQUES
Abstract:
(EN) Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner.
(FR) La présente invention concerne des procédés de séquençage par liaison de détection de nucléotides apparentés en utilisant une enzyme de polymérisation d’ADN invalidé qui possède la capacité de se lier au prochain nucléotide correct en aval d’une amorce d’une manière dépendante du modèle, mais ne possède pas l’activité requise pour favoriser la formation d’une liaison phosphodiester. L’utilisation de l’ADN polymérase invalidée permet l’interrogation d’un nucléotide à la fois, sans incorporation d’un quelconque nucléotide. Les nucléotides marqués, tels que les nucléotides marqués de manière fluorescente, peuvent être utilisés en conjonction avec l’ADN polymérase invalidée pour établir une identité nucléotidique apparentée d’une manière rapide.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)