Search International and National Patent Collections

1. (WO2017189894) MULTIPLEX GENOME ENGINEERING IN EUKARYOTES

Pub. No.:    WO/2017/189894    International Application No.:    PCT/US2017/029922
Publication Date: Fri Nov 03 00:59:59 CET 2017 International Filing Date: Fri Apr 28 01:59:59 CEST 2017
IPC: C12N 15/10
Applicants: YALE UNIVERSITY
Inventors: BARBIERI, Edward
ISAACS, Farren
Title: MULTIPLEX GENOME ENGINEERING IN EUKARYOTES
Abstract:
Compositions and methods for gene editing are provided. The methods employ an oligo-based annealing mechanism that is rooted in the process of DNA replication rather than homologous recombination (HR). Oligo incorporation efficiencies are comparable and often exceed those of CRISPR/cas9 editing without the need for double strand breaks (DSBs). By relying on the multiplex annealing of oligos rather than DSBs the process is highly scalable across a genomic region of interest and can generate many scarless modifications of a chromosome simultaneously. Combinatorial genomic diversity can be generated across a population of cells in a single transformation event; genomic landscapes can be traversed through successive iterations of the process, and genome-wide changes can be massively parallelized and amplified through systematic strain mating.