Search International and National Patent Collections

1. (WO2017189632) SYSTEMS, DEVICES AND METHODS FOR SEQUENTIAL ANALYSIS OF COMPLEX MATRIX SAMPLES FOR HIGH CONFIDENCE BACTERIAL DETECTION AND DRUG SUSCEPTIBILITY PREDICTION USING A FLOW CYTOMETER

Pub. No.:    WO/2017/189632    International Application No.:    PCT/US2017/029492
Publication Date: Fri Nov 03 00:59:59 CET 2017 International Filing Date: Wed Apr 26 01:59:59 CEST 2017
IPC: G01N 33/493
G01N 15/14
G01N 15/10
Applicants: APERTURE BIO, LLC
Inventors: GOMBRICH, Matthew, D.
RAMSAROOP, Shawn
BOZZUTI, Margaret
Title: SYSTEMS, DEVICES AND METHODS FOR SEQUENTIAL ANALYSIS OF COMPLEX MATRIX SAMPLES FOR HIGH CONFIDENCE BACTERIAL DETECTION AND DRUG SUSCEPTIBILITY PREDICTION USING A FLOW CYTOMETER
Abstract:
Method for testing a sample of body fluid for the presence of bacteria and comprising the steps of adjusting the dilution of the sample to a predetermined concentration, of dividing the diluted sample into two batches defining a baseline-batch and a control-batch, of testing the baseline-batch at a time T0 with the flow cytometer to obtain enumerative baseline bacterial values, of culturing the control-batch in growth media between times T0 and T1, of testing the control-batch at time T1 with the flow cytometer to obtain enumerative control bacterial values, and of comparing the control values to the baseline values to determine a bacteria growth-ratio.