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1. (WO2017189336) METHODS AND COMPOSITIONS FOR GENOMIC EDITING

Pub. No.:    WO/2017/189336    International Application No.:    PCT/US2017/028676
Publication Date: Fri Nov 03 00:59:59 CET 2017 International Filing Date: Fri Apr 21 01:59:59 CEST 2017
IPC: C12N 15/09
C12N 15/79
C12N 5/10
C12N 15/90
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: THORNER, Jeremy W.
FINNIGAN, Gregory Charles
Title: METHODS AND COMPOSITIONS FOR GENOMIC EDITING
Abstract:
The present disclosure provides genetically modified cells and non-human organisms, and methods of producing such cells and organisms. Also provided are methods of editing the genome of such cells and organisms. The cells and non-human organisms of the disclosure are genetically modified such that their genome includes an integrated heterologous nucleic acid (that includes a CRISPR/Cas target sequence) at one or more (e.g., 2 or more, 3 or more, 4 or more, etc.) positions within the genome. The integrated nucleic acids, which include the same CRISPR/Cas target sequence, allow for simultaneous gene editing at multiple different positions within a genome using a single species of CRISPR/Cas guide RNA (i.e., one guide RNA will target multiple sites because the multiple sites have the same target sequence).