KR2017004610 METHOD FOR EVALUATING,<i> IN VIVO</i>, ACTIVITY OF RNA-GUIDED NUCLEASE IN HIGH-THROUGHPUT MANNER

1. (WO2017188797) METHOD FOR EVALUATING, IN VIVO, ACTIVITY OF RNA-GUIDED NUCLEASE IN HIGH-THROUGHPUT MANNER

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Pub. No.:		WO/2017/188797		International Application No.:		PCT/KR2017/004610
Publication Date:		02.11.2017		International Filing Date:		28.04.2017

IPC:

C12Q 1/68 (2006.01), C12Q 1/42 (2006.01), C12N 15/63 (2006.01), C12N 9/22 (2006.01), G06F 19/22 (2011.01)

Applicants:

INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY [KR/KR]; 50, Yonsei-ro, Seodaemun-gu, Seoul 03722 (KR)

Inventors:

KIM, Hyong Bum; (KR).
KIM, Hui Kwon; (KR).
SONG, Myung Jae; (KR)

Agent:

SON, Min; (KR)

Priority Data:

10-2016-0052365

28.04.2016

Title

(EN) METHOD FOR EVALUATING, IN VIVO, ACTIVITY OF RNA-GUIDED NUCLEASE IN HIGH-THROUGHPUT MANNER
(FR) PROCÉDÉ D'ÉVALUATION, IN VIVO, DE L'ACTIVITÉ D'UNE NUCLÉASE GUIDÉE PAR ARN DE MANIÈRE TRÈS EFFICACE
(KO) IN VIVO에서 RNA-가이드 뉴클레아제의 활성을 고처리량 방식으로 평가하는 방법

Abstract:

(EN)The present invention relates to a method for evaluating, in vivo, the activity of an RNA-guided nuclease in a high-throughput manner and, more particularly, to a method for evaluating the activity of an RNA-guided nuclease by using indel frequencies of a cell library comprising separate oligonucleotides including a guide RNA-encoding base sequence and a target base sequence. According to the present invention, an RNA-guided nuclease characteristic analysis method using a library of guide RNA-target sequence pairs can evaluate, in vivo, the activity of the RNA-guided nuclease in a high-throughput manner, thereby being very useful in all fields in which an RNA-guided nuclease is applied.
(FR)La présente invention concerne un procédé d'évaluation, in vivo, de l'activité d'une nucléase guidée par ARN de manière très efficace et, plus particulièrement, un procédé d'évaluation de l'activité d'une nucléase guidée par ARN en utilisant les fréquences d’insertion/de délétion (indel) d'une bibliothèque de cellules comprenant des oligonucléotides séparés comprenant une séquence de base de codage d'ARN de guidage et une séquence de base cible. Selon la présente invention, un procédé d'analyse de caractéristique de nucléase guidée par ARN utilisant une bibliothèque de paires de séquences cibles ARN guidée peut évaluer, in vivo, l'activité de la nucléase guidée par ARN de manière très efficace, étant ainsi très utile dans tous les domaines dans lesquels une nucléase guidée par ARN est appliquée.
(KO)본 발명은 세포에서 RNA-가이드 뉴클레아제 (RNA-guided nuclease)의 활성을 고처리량 방식 (high-throughput manner)으로 평가하는 방법에 관한 것으로, 구체적으로 가이드 RNA (guide RNA) 코딩 염기서열 및 표적 염기서열을 포함하는 분리된 올리고뉴클레오티드를 포함하는, 세포 라이브러리의 인델 빈도 (indel frequency)로부터 RNA-가이드 뉴클레아제의 활성을 평가하는 방법에 관한 것이다. 본 발명의 가이드 RNA-표적서열 페어 (pair) 라이브러리를 이용한 RNA-가이드 뉴클레아제의 특성을 분석하는 방법은 세포 내 (in vivo)에서 고처리량 방식 (high-throughput manner)으로 RNA-가이드 뉴클레아제의 활성을 평가할 수 있도록 하므로, RNA-가이드 뉴클레아제를 적용하는 모든 분야에서 매우 유용하게 활용될 수 있다.

Designated States:

AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).

Publication Language:	Korean (KO)
Filing Language:	Korean (KO)

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