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1. (WO2017187656) ASSAY METHOD FOR ANTI-DRUG ANTIBODIES

Pub. No.:    WO/2017/187656    International Application No.:    PCT/JP2016/082135
Publication Date: Fri Nov 03 00:59:59 CET 2017 International Filing Date: Sat Oct 29 01:59:59 CEST 2016
IPC: G01N 33/53
A61P 1/04
G01N 33/543
A61K 39/395
A61P 19/02
A61P 29/00
Applicants: JIMRO CO., LTD.
株式会社JIMRO
Inventors: KASHIWAGI, Nobuhito
柏木 伸仁
SAITOH, Fumio
齋藤 史郎
KANEDA, Kenta
金田 健太
MAEGAWA, Hidetaka
前川 豪孝
YAGIHASHI, Yuko
八木橋 裕子
Title: ASSAY METHOD FOR ANTI-DRUG ANTIBODIES
Abstract:
Provided is a method for more simply and accurately measuring ADAs that develop in a patient receiving drug-based, molecular-targeted therapy, or the like. This assay method for ADAs in an assay sample includes the following steps (1) to (4). (1) A step for preparing a specimen which includes an assay sample of ADAs; (2) a step for bringing the specimen prepared in step (1) into contact with a support on which complement C1q, an anti-C3d antibody, or an anti-C1q antibody has been immobilized; (3) a step for bringing a labeled or unlabeled antibody to the drug corresponding to the aforementioned ADAs into contact with the solid-phase support produced in step (2); and (4) a step for measuring the quantity of drug-ADA immune complexes bound to complement C1q, the anti-C3d antibody, or the anti-C1q antibody.