Provided is a surface enhanced Raman scattering technique based detection chip, comprising: a nano metal substrate, two or more signal molecules, and two or more detection objects for detecting tumour markers, wherein the signal molecules are uniformly distributed on the substrate, and the detection objects are respectively fixed onto different signal molecules. Also provided are a preparation method for a detection chip, and a kit containing same, and a tumour detection method. By fixing different Raman signal molecules to different micro zones, synchronous and quantitative detection of a plurality of tumour markers are realized by using a nano-stress sensing technique, having a tremendous application potential.