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1. (WO2017185289) METHOD FOR PREPARING ACTIVE EXTRACT OF SEA BUCKTHORN

Pub. No.:    WO/2017/185289    International Application No.:    PCT/CN2016/080485
Publication Date: Fri Nov 03 00:59:59 CET 2017 International Filing Date: Fri Apr 29 01:59:59 CEST 2016
IPC: A61K 36/185
A61P 29/00
A61P 31/00
A61P 35/00
Applicants: JIN, Yinghua
金英花
Inventors: JIN, Yinghua
金英花
Title: METHOD FOR PREPARING ACTIVE EXTRACT OF SEA BUCKTHORN
Abstract:
A method for preparing an active extract of sea buckthorn comprises the following steps: pulverizing sea buckthorn, adding water into the pulverized sea buckthorn to mix evenly, putting the mixture in a sealed state for 1 to 3 days, air-drying the mixture in an indoor temperature, carrying ultrasonic extraction by sequentially using an alcohol solution, normal butyl alcohol and acetone, the extraction temperature ranging from 70ºC to 80 ºC, and the time of a single ultrasonic extraction ranging from 5 h to 10 h; merging the extracted liquids to obtain sea buckthorn extract solution; carrying filtering when the sea buckthorn extract solution is hot, and feeding the sea buckthorn extract solution into a centrifugal machine to carry out centrifugation, the rotation speed of the centrifugal machine being 6000 r/min to 7000 r/min, and the centrifugation time ranging from 30 min to 50 min; collecting the supernatant; carrying out pumping filtration, and feeding the filtrate into a rotary evaporator for concentration, the concentration temperature ranging from 50ºC to 60ºC; and adding alcohol to mix evenly, and then adding water to make the concentration of the alcohol range from 5 wt% to 15 wt%, and carrying out sterilization and sealed preservation, so as to obtain the active extract of sea buckthorn. The preparation method has a simple process, low costs and high extraction efficiency, and has promotion and application value.