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1. (WO2017184998) A DEVICE FOR HIGH-THROUGHPUT MULTI-PARAMETER FUNCTIONAL PROFILING OF THE SAME CELLS IN MULTICELLULAR SETTINGS AND IN ISOLATION

Pub. No.:    WO/2017/184998    International Application No.:    PCT/US2017/028884
Publication Date: Fri Oct 27 01:59:59 CEST 2017 International Filing Date: Sat Apr 22 01:59:59 CEST 2017
IPC: C12Q 1/02
G01N 33/48
Applicants: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Inventors: KELBAUSKAS, Laimonas
GLENN, Honor
HOUKAL, Jeff
ANDERSON, Clifford
TIAN, Yanqing
SU, Fengyu
MELDRUM, Deirdre
Title: A DEVICE FOR HIGH-THROUGHPUT MULTI-PARAMETER FUNCTIONAL PROFILING OF THE SAME CELLS IN MULTICELLULAR SETTINGS AND IN ISOLATION
Abstract:
A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1 ) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.