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1. (WO2017183655) METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/183655    International Application No.:    PCT/JP2017/015704
Publication Date: 26.10.2017 International Filing Date: 19.04.2017
Chapter 2 Demand Filed:    19.09.2017    
IPC:
C12N 5/071 (2010.01), A61K 35/36 (2015.01), A61L 27/00 (2006.01)
Applicants: KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION [JP/JP]; 465, Kajii-cho, Kawaramachi-dori Hirokoji-agaru, Kamigyo-ku, Kyoto-shi, Kyoto 6028566 (JP)
Inventors: NAKAMURA, Takahiro; (JP).
KINOSHITA, Shigeru; (JP).
YOKOO, Seiichi; (JP).
YAMAGAMI, Satoru; (JP)
Agent: SAEGUSA & PARTNERS; Kitahama TNK Building, 1-7-1, Doshomachi, Chuo-ku, Osaka-shi, Osaka 5410045 (JP)
Priority Data:
2016-084471 20.04.2016 JP
Title (EN) METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET
(FR) PROCÉDÉ DE PRODUCTION DE FEUILLE DE CELLULES ÉPITHÉLIALES CULTIVÉES
(JA) 培養上皮シートの製造方法
Abstract: front page image
(EN)A method for producing an epithelial cell sheet, said method comprising a step for cultivating cells, which are derived from oral mucosal epithelial cells, in a serum-free medium on a substrate, wherein the serum-free medium contains: (i) EGF protein or KGF protein; (ii) a B-27 supplement; and (iii) an ROCK inhibitor.
(FR)L'invention concerne un procédé de production d'une feuille de cellules épithéliales, ledit procédé comprenant une étape de culture de cellules issues de cellules épithéliales de la muqueuse buccale, dans un milieu exempt de sérum sur un substrat, ce milieu contenant : (i) une protéine EGF ou une protéine KGF ; (ii) un supplément de B-27 ; et (iii) un inhibiteur de ROCK.
(JA)口腔粘膜上皮細胞由来の細胞を、無血清培地中、基材上で培養する工程を含む上皮細胞シートの製造方法であって、前記無血清培地が(i)EGFタンパク質またはKGFタンパク質、(ii)B-27サプリメント、及び(iii)ROCK阻害剤を含む無血清培地である、方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)