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1. (WO2017182404) METHOD FOR SEQUENCING OLIGOSACCHARIDES
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Pub. No.: WO/2017/182404 International Application No.: PCT/EP2017/059050
Publication Date: 26.10.2017 International Filing Date: 14.04.2017
IPC:
G01N 21/35 (2014.01) ,A61K 31/702 (2006.01) ,C07H 3/06 (2006.01) ,G01N 33/66 (2006.01) ,H01J 49/26 (2006.01) ,G06F 19/00 (2011.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
17
Systems in which incident light is modified in accordance with the properties of the material investigated
25
Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31
Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35
using infra-red light
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31
Medicinal preparations containing organic active ingredients
70
Carbohydrates; Sugars; Derivatives thereof
702
Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3
Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06
Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
66
involving blood sugars, e.g. galactose
H ELECTRICITY
01
BASIC ELECTRIC ELEMENTS
J
ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
49
Particle spectrometers or separator tubes
26
Mass spectrometers or separator tubes
G PHYSICS
06
COMPUTING; CALCULATING; COUNTING
F
ELECTRIC DIGITAL DATA PROCESSING
19
Digital computing or data processing equipment or methods, specially adapted for specific applications
Applicants:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE [FR/FR]; 3 rue Michel Ange 75794 PARIS CEDEX 16, FR
UNIVERSITE CLAUDE BERNARD LYON 1 [FR/FR]; 43 boulevard du 11 novembre 1918 69100 VILLEURBANNE, FR
Inventors:
COMPAGNON, Isabelle; FR
SCHINDLER, Baptiste; FR
Agent:
LTL SAS; 56 BOULEVARD NIELS BOHR CS 52132 69100 VILLEURBANNE, FR
Priority Data:
165342518.04.2016FR
Title (EN) METHOD FOR SEQUENCING OLIGOSACCHARIDES
(FR) PROCÉDÉ DE SÉQUENÇAGE D'OLIGOSACCHARIDES
Abstract:
(EN) The present application describes a method for sequencing oligosaccharides, which enables the primary sequence of an oligosaccharide with unknown structure to be identified, including the composition of same in monosaccharides, the position (regiochemistry) and configuration (stereochemistry) of the glycosidic bonds, the nature and position of the functional modifications, and the branched structure thereof, including in particular identifying the reductive end. Said method of sequencing oligosaccharides comprises the steps of i. fragmentation of the oligosaccharides into disaccharides and monosaccharides while preserving the molecular structure of the constituents as present in the oligosaccharide to be sequenced, ii. separating each previously obtained disaccharide and monosaccharide by mass spectrometry, iii. analysing, by vibrational IR (infrared) spectroscopy, each previously separated disaccharide and monosaccharide, iv. identifying the structure of each disaccharide and monosaccharide by comparing the IR spectra obtained with a set of IR spectra of reference disaccharides and monosaccharides, and v. defining the sequence of the oligosaccharide by combining the structures identified for each disaccharide and monosaccharide.
(FR) La présente demande décrit un procédé de séquençage d'oligosaccharides, qui permet d'identifier la séquence primaire d'un oligosaccharide de structure inconnue, incluant sa composition en monosaccharides, la position (régiochimie) et la configuration (stéréochimie) des liaisons glycosidiques, la nature et la position des modifications fonctionnelles, et sa structure branchée, dont en particulier l'identification de l'extrémité réductrice. Ce procédé de séquençage d'oligosaccharides comprend les étapes de i. fragmentation des oligosaccharides en disaccharides et monosaccharides en préservant la structure moléculaire des constituants telle que présente dans l'oligosaccharide à séquencer, ii. séparation de chaque disaccharide et monosaccharide obtenus précédemment par spectrométrie de masse, iii. analyse par spectroscopie vibrationnelle IR (infrarouge) de chaque disaccharide et monosaccharide séparés précédemment, iv. identification de la structure de chaque disaccharide et monosaccharide par comparaison des spectres IR obtenus à un ensemble de spectres IR de disaccharides et monosaccharides de référence, et v. définition de la séquence de l'oligosaccharide par combinaison des structures identifiées pour chaque disaccharide et monosaccharide.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: French (FR)
Filing Language: French (FR)