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1. (WO2017181880) METHOD FOR CONSTRUCTING DNA SEQUENCING LIBRARY FOR TO-BE-DETECTED GENOME, AND APPLICATIONS THEREOF

Pub. No.:    WO/2017/181880    International Application No.:    PCT/CN2017/080142
Publication Date: Fri Oct 27 01:59:59 CEST 2017 International Filing Date: Wed Apr 12 01:59:59 CEST 2017
IPC: C12N 15/10
C40B 50/06
C40B 60/14
C12Q 1/68
Applicants: TSINGHUA UNIVERSITY
清华大学
Inventors: XIE, Wei
颉伟
ZHANG, Bingjie
张冰洁
Title: METHOD FOR CONSTRUCTING DNA SEQUENCING LIBRARY FOR TO-BE-DETECTED GENOME, AND APPLICATIONS THEREOF
Abstract:
Provided is a method for constructing a DNA sequencing library for a to-be-detected genome. The method comprises: (1), carrying out first digestion processing on a to-be-detected genome by using micrococcal nuclease, so as to obtain a first digestion processing product; (2), carrying out co-immunoprecipitation processing on the first digestion processing product, so as to obtain a co-immunoprecipitation processing product; (3), carrying out second digestion processing on the co-immunoprecipitation processing product by using protease K, so as to obtain second digestion processing product; (4), directly carrying out dephosphorylation processing on the second digestion processing product by using shrimp alkaline phosphatase, so as to obtain a dephosphorylation processing product; (5), directly carrying out denaturalization processing on the dephosphorylation processing product, so as to obtain a denaturalization processing product containing single-stranded DNA molecules; and (6), based on the denaturalization processing product containing single-stranded DNA molecules, obtaining a sequencing library according to a TELP method.