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1. (WO2017181809) PENICILLIN EXPANDASE MUTANT, DNA ENCODING MUTANT, AND REAGENT KIT CONTAINING MUTANT AND USE THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/181809 International Application No.: PCT/CN2017/077858
Publication Date: 26.10.2017 International Filing Date: 23.03.2017
IPC:
C12N 9/02 (2006.01) ,C12N 15/53 (2006.01) ,C12P 35/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
53
Oxidoreductases (1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
35
Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
Applicants: LUI, Yuk Sun[CN/CN]; CN (BZ)
BIORIGHT WORLDWIDE CO., LTD.[GB/GB]; Virgin Islands TrustNet Chambers, P.O. Box 3444, Road Town Tortola, VG
Inventors: LUI, Yuk Sun; CN
CHENG, Shiu Ming; CN
SIU, Yau Lung; CN
WANG, Jun; CN
Agent: TEE&HOWE INTELLECTUAL PROPERTY ATTORNEYS; DING, Yeping 10th Floor, Tower D, Minsheng Financial Center, 28 Jianguomennei Avenue, Dongcheng District Beijing 100005, CN
Priority Data:
201610239821.X18.04.2016CN
Title (EN) PENICILLIN EXPANDASE MUTANT, DNA ENCODING MUTANT, AND REAGENT KIT CONTAINING MUTANT AND USE THEREOF
(FR) EXPANDASE DE PÉNICILLINE MUTANTE, ADN CODANT POUR LA MUTANTE, ET TROUSSE DE RÉACTIF CONTENANT LA MUTANTE ET SON UTILISATION
(ZH) 青霉素扩环酶突变体、编码该突变体的DNA、含有该突变体的试剂盒及其应用
Abstract:
(EN) Provided is a penicillin expandase mutant, wherein same takes SEQ ID NO: 2 of the sequence listing as the reference sequence, and has the mutations of at least one of threonine at position 42 and glutamine at position 126. Also provided are the DNA encoding the mutant, and the reagent kit containing the mutant and the use thereof. The penicillin expandase mutant has an improved catalytic activity and heat resisting property.
(FR) La présente invention décrit une expandase de pénicilline mutante, ce dernier prenant SEQ ID no : 2 de la liste des séquences comme séquence de référence, et possédant des mutations de la thréonine à la position 42 et/ou de la glutamine à la position 126. Sont également décrits l’ADN codant pour ladite mutante, et la trousse de réactif contenant ladite mutante et son utilisation. L'expandase de pénicilline mutante présente une activité catalytique et une propriété de résistance à la chaleur améliorées.
(ZH) 提供了青霉素扩环酶突变体,是以序列表SEQ ID NO:2为参考序列,具有位于第42位苏氨酸和第126位谷氨酰胺中至少一者的突变。还提供了编码该突变体的DNA、含有该突变体的试剂盒及其应用。该青霉素扩环酶突变体具有提高的催化活性和耐热性。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)