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|1. (WO2017177981) RENATURATION AND PURIFICATION METHODS FOR RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR|
|Applicants:||JIANGSU HENGRUI MEDICINE CO., LTD.
|Title:||RENATURATION AND PURIFICATION METHODS FOR RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR|
Renaturation and purification methods for a recombinant human granulocyte colony-stimulating factor. By eliminating a reducing agent before the renaturation in a buffer solution substitution manner, the controllability and the renaturation rate of the renaturation process are improved, and the constitution of the renaturation buffer solution is optimized. The method is applicable to large-scale industrial expanded production, has low requirements on devices, is easy to operate, allows an obtained protein to have a high purity and good stability, and can reduce side-effects of a final product in clinic, and can improve the safety and the effectiveness of a drug.