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1. (WO2017177981) RENATURATION AND PURIFICATION METHODS FOR RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR

Pub. No.:    WO/2017/177981    International Application No.:    PCT/CN2017/080656
Publication Date: Fri Oct 20 01:59:59 CEST 2017 International Filing Date: Sat Apr 15 01:59:59 CEST 2017
IPC: C07K 14/535
C07K 1/113
C07K 1/107
Applicants: JIANGSU HENGRUI MEDICINE CO., LTD.
江苏恒瑞医药股份有限公司
Inventors: ZHANG, Chenguang
张晨光
WANG, Hongwei
王宏伟
XU, Feng
徐峰
WANG, Jun
汪军
QI, Yanyan
齐艳艳
Title: RENATURATION AND PURIFICATION METHODS FOR RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR
Abstract:
Renaturation and purification methods for a recombinant human granulocyte colony-stimulating factor. By eliminating a reducing agent before the renaturation in a buffer solution substitution manner, the controllability and the renaturation rate of the renaturation process are improved, and the constitution of the renaturation buffer solution is optimized. The method is applicable to large-scale industrial expanded production, has low requirements on devices, is easy to operate, allows an obtained protein to have a high purity and good stability, and can reduce side-effects of a final product in clinic, and can improve the safety and the effectiveness of a drug.