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1. (WO2017176796) COMPOSITIONS AND METHODS FOR PREVENTING AND TREATING DISEASE

Pub. No.:    WO/2017/176796    International Application No.:    PCT/US2017/025995
Publication Date: Fri Oct 13 01:59:59 CEST 2017 International Filing Date: Wed Apr 05 01:59:59 CEST 2017
IPC: C07K 14/76
C07K 14/765
C12N 1/20
A61K 39/10
A61K 39/02
Applicants: UNIVERSITY OF VIRGINIA PATENT FOUNDATION
Inventors: HEWLETT, Erik, L.
GRAY, Mary, C.
GONYAR, Laura, A.
Title: COMPOSITIONS AND METHODS FOR PREVENTING AND TREATING DISEASE
Abstract:
A method for increasing production of a polypeptide in a bacterial cell in culture is provided. The method can comprise contacting a bacterial cell in a culture medium with an amount of an albumin polypeptide sufficient to induce an increase in accumulation of the polypeptide in the bacterial cell and/or an increase in secretion of the polypeptide from the bacterial cell into the culture medium relative to a bacterial cell grown in a culture medium in the absence of the albumin polypeptide. The bacterial cell can be a Bordetella species cell, optionally wherein the Bordetella species cell is a Bordetella pertussis cell, a Bordetella bronchiseptica cell or a Bordetella parapertussis cell. Kits, vaccine production methods, screening methods and therapeutic methods are also disclosed.