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1. (WO2017176541) ENZYMATIC NUCLEIC ACID SYNTHESIS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/176541    International Application No.:    PCT/US2017/024939
Publication Date: 12.10.2017 International Filing Date: 30.03.2017
IPC:
C40B 50/18 (2006.01), C12P 19/30 (2006.01), C12Q 1/68 (2006.01)
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US/US]; 17 Quincy Street Cambridge, Massachusetts 02138 (US)
Inventors: LEE, Henry H.; (US).
CHURCH, George M.; (US).
KALHOR, Reza; (US)
Agent: IWANICKI, John P.; (US)
Priority Data:
62/317,919 04.04.2016 US
Title (EN) ENZYMATIC NUCLEIC ACID SYNTHESIS
(FR) SYNTHÈSE ENZYMATIQUE D'ACIDES NUCLÉIQUES
Abstract: front page image
(EN)The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3' terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3' terminal nucleotide such that the selected nucleotide becomes a 3' terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
(FR)L'invention concerne des procédés de fabrication d'un polynucléotide dans lequel l'addition de nucléotides peut être commandée physiquement, chimiquement et/ou enzymatiquement. Les procédés consistent à combiner un certain nucléotide, des cations, une ADN polymérase sensible aux erreurs ou indépendante de la matrice au niveau d'un site de réaction comprenant une séquence d'initiation fixée à celui-ci et ayant un nucléotide de terminaison 3', les réactifs de réaction pouvant être modulés et dans des conditions qui permettent une addition covalente d'un ou de plusieurs d'un certain nucléotide au nucléotide à terminaison 3' de telle sorte que ledit certain nucléotide devienne un nucléotide à terminaison 3', et répéter l'étape d'addition jusqu'à ce que le polynucléotide soit formé.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)