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1. (WO2017175072) PEPTIDE SHUTTLE BASED GENE DISRUPTION

Pub. No.:    WO/2017/175072    International Application No.:    PCT/IB2017/000512
Publication Date: Fri Oct 13 01:59:59 CEST 2017 International Filing Date: Sat Apr 08 01:59:59 CEST 2017
IPC: C12N 15/09
A61K 35/12
A61K 35/14
A61K 35/28
A61K 38/17
A61K 48/00
C12N 15/00
C12N 15/87
C12N 15/90
C12N 9/22
Applicants: FELDAN BIO INC.
Inventors: GUAY, David
DEL'GUIDICE, Thomas
LEPETIT-STOFFAES, Jean-pascal
Title: PEPTIDE SHUTTLE BASED GENE DISRUPTION
Abstract:
The present description relates to synthetic peptides useful for increasing the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells. More specifically, the present description relates to synthetic peptides and polypeptide-based shuttle agents comprising an endosome leakage domain (ELD) operably linked to a cell penetrating domain (CPD), or an ELD operably linked to a histidine-rich domain and a CPD. Compositions, kits, methods and uses relating to same are also described. Methods for using the disclosed polypeptide-based shuttles for delivering a transcription factor and/or nuclease to eukaryotic cell nucleus and uses of the derived cells are also described.