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1. (WO2017173696) EX VIVO EXPANSION METHOD FOR CORD BLOOD NK CELL, AND KIT AND APPLICATION THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2017/173696 International Application No.: PCT/CN2016/081198
Publication Date: 12.10.2017 International Filing Date: 06.05.2016
IPC:
C12N 5/0783 (2010.01) ,A61K 35/17 (2015.01) ,A61P 35/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0783
T cells; NK cells; Progenitors of T or NK cells
[IPC code unknown for A61K 35/17]
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35
Antineoplastic agents
Applicants: BEIJING JINGMENG STEM CELL TECHNOLOGY CO., LTD.[CN/CN]; F6, JMGK Building B No. 5-2 Shangdi East Road, Haidian District Beijing 100085, CN
Inventors: WU, Xiaoyun; CN
LIU, Xuemin; CN
KANG, Huiyan; CN
WANG, Yunhong; CN
FAN, Xiaoxiang; CN
LI, Zhigang; CN
DING, Wei; CN
WU, Lihua; CN
LI, Jinyan; CN
SHEN, Dianfu; CN
ZHANG, Huizhen; CN
Agent: SHANGCHENG & PARTNERS; Room 1801, 18th Floor Ever Bright World Center Building, No. 28 West Ping An Li Avenue, Xicheng District Beijing 100034, CN
Priority Data:
201610214351.107.04.2016CN
Title (EN) EX VIVO EXPANSION METHOD FOR CORD BLOOD NK CELL, AND KIT AND APPLICATION THEREOF
(FR) PROCÉDÉ D’EXPANSION EX VIVO DESTINÉ AUX CELLULES NK DU SANG OMBILICAL, ET TROUSSE ET APPLICATION ASSOCIÉES
(ZH) 脐血NK细胞的体外扩增方法及其试剂盒与应用
Abstract:
(EN) Provides an ex vivo expansion method for a cord blood NK cell, and a kit and application thereof. The ex vivo expansion method for the cord blood NK cell comprises: separating a cord blood monocyte; activating, culturing, and proliferation; and culturing of the cord blood NK cell. The invention does not require cell sorting and does not require a trophoblast. A reagent in the kit does not contain animal-based component. The invention provides a high production volume of the NK cell. The cord blood NK cell obtained has at least 90% purity, a total cell count of up to 1010-11 per part of cord blood, and can kill a tumor cell effectively.
(FR) La présente invention décrit un procédé d’expansion ex vivo destiné à une cellule NK du sang ombilical, et une trousse et une application associées. Le procédé d’expansion ex vivo concernant la cellule NK du sang ombilical comprend : la séparation d’un monocyte de sang du cordon ; l’activation, la culture, et la prolifération ; et la culture de la cellule NK du sang du cordon. L’invention ne nécessite pas de tri des cellules et ne nécessite pas de trophoblaste. Un réactif dans la trousse ne contient pas de constituant provenant d’un animal. L’invention fournit un volume de production élevé de la cellule NK. La cellule NK du sang du cordon obtenue présente au moins 90 % de pureté, une numération totale des cellules allant jusqu’à 1010-11 par partie de sang du cordon, et peut tuer de manière efficace une cellule tumorale.
(ZH) 提供脐血NK细胞的体外扩增方法及其试剂盒与应用,脐血NK细胞的体外扩增方法包括分离的脐血单个核细胞、活化培养和增殖培养脐血NK细胞。本发明不需要细胞分选,不需要滋养层细胞,所用试剂不含动物源成分,NK细胞产量高,获得的脐血NK细胞的纯度达90%以上,细胞总数可达10 10-11个/份脐血,对肿瘤细胞的杀伤力高。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)