WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2017172772) METHODS OF HEAT INACTIVATION OF ADENOVIRUS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/172772 International Application No.: PCT/US2017/024545
Publication Date: 05.10.2017 International Filing Date: 28.03.2017
IPC:
C12N 15/861 (2006.01) ,C12N 15/864 (2006.01) ,C12N 7/04 (2006.01)
Applicants: DIMENSION THERAPEUTICS[US/US]; 840 Memorial Drive, 4th Floor Cambridge, MA 02139, US
Inventors: MORRISON, Christopher, J.; US
MARATT, James, D.; US
Agent: DELFINO, Wyatt; US
BRODOWSKI, Michael; US
ARGENTIERI, Steven, R.; US
ASHRAF, Shovon; US
BUCHMAN, Joshua, J.; US
Priority Data:
62/314,11628.03.2016US
Title (EN) METHODS OF HEAT INACTIVATION OF ADENOVIRUS
(FR) PROCÉDÉS D'INACTIVATION THERMIQUE D'ADÉNOVIRUS
Abstract: front page image
(EN) The present disclosure generally relates to methods of protecting the genomic integrity and/or biological activity of AAV viral particles in a sample containing both AAV particles and helper virus particles during heat inactivation. The methods include heating, to a temperature greater than or equal to 45°C, a sample containing helper virus particles, AAV particles, and a buffer. The buffer includes a concentration of 10 mM or greater kosmotropic salts and/or a concentration of 10 mM or greater of divalent or trivalent cations.
(FR) La présente invention concerne, de manière générale, des procédés de protection de l'intégrité génomique et/ou de l'activité biologique de particules virales d'AAV dans un échantillon contenant à la fois des particules d'AAV et des particules de virus auxiliaire pendant l'inactivation thermique. Les procédés comprennent le chauffage, à une température supérieure ou égale à 45°C, d'un échantillon contenant des particules de virus auxiliaire, des particules d'AAV et un tampon. Le tampon comprend une concentration de 10 mM ou plus en sels cosmotropes et/ou une concentration de 10 mM ou plus en cations divalents ou trivalents.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)