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1. (WO2017171606) METHOD FOR THE DETECTION OF MOLECULES BEING IN CLOSE PROXIMITY
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Pub. No.: WO/2017/171606 International Application No.: PCT/SE2017/050187
Publication Date: 05.10.2017 International Filing Date: 01.03.2017
IPC:
G01N 33/557 (2006.01) ,G01N 33/542 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
557
using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
536
with immune complex formed in liquid phase
542
with steric inhibition or signal modification, e.g. fluorescent quenching
Applicants:
RIDGEVIEW INSTRUMENTS AB [SE/SE]; Skillsta 4 740 20 VÄNGE, SE
Inventors:
ANDERSSON, Karl; SE
BONDZA, Sina; SE
BUIJS, Josephus; SE
Agent:
BRANN AB; Box 3690 103 59 STOCKHOLM, SE
Priority Data:
1650399-729.03.2016SE
Title (EN) METHOD FOR THE DETECTION OF MOLECULES BEING IN CLOSE PROXIMITY
(FR) PROCÉDÉ DE DÉTECTION DE MOLÉCULES SE TROUVANT À PROXIMITÉ IMMÉDIATE
Abstract:
(EN) The invention relates to a method for determining if two species are located in close proximity to each other. It comprises immobilization of an object 111 to a solid support 110, contacting the solid support with a labelled first species dissolved in liquid 120, measuring the rate of formation of complex of object and species 151, supplementing the liquid with a quenching second species 121, measuring the rate of formation of complex of object and first species in the presence of second species 152, followed by determining change in rate of complex formation before and after addition of second species. A reduction of the rate of complex formation due to the addition of second species indicates that first and second species are located in close proximity to each other when binding to the object. An exemplary application area comprises biological objects like cells and macromolecular species.
(FR) L'invention concerne un procédé permettant de déterminer si deux espèces sont situées à proximité immédiate l'une de l'autre. Le procédé consiste : à immobiliser un objet (111) sur un support solide (110), à mettre en contact le support solide avec une première espèce marquée dissoute dans un liquide (120), à mesurer le taux de formation d'un complexe de l'objet et de l'espèce (151), à ajouter au liquide une seconde espèce de trempe (121), à mesurer la vitesse de formation d'un complexe de l'objet et de la première espèce en présence d'une seconde espèce (152), puis à déterminer un changement de taux de formation de complexes avant et après l'ajout de la seconde espèce. Une réduction du taux de formation de complexes en raison de l'ajout de la seconde espèce indique que des première et seconde espèces sont situées à proximité immédiate l'une de l'autre lorsqu'elles se lient à l'objet. Une zone d'application exemplaire comprend des objets biologiques tels que des cellules et des espèces macromoléculaires.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)