Search International and National Patent Collections

1. (WO2017170771) METHOD FOR INTRODUCING Cas9 PROTEIN INTO FERTILIZED EGG OF MAMMAL

Pub. No.:    WO/2017/170771    International Application No.:    PCT/JP2017/013061
Publication Date: Fri Oct 06 01:59:59 CEST 2017 International Filing Date: Thu Mar 30 01:59:59 CEST 2017
IPC: C12N 15/09
A01K 67/027
C12N 5/071
C12N 13/00
Applicants: TOKUSHIMA UNIVERSITY
国立大学法人徳島大学
OSAKA UNIVERSITY
国立大学法人大阪大学
BEX CO., LTD.
株式会社ベックス
Inventors: HASHIMOTO, Masakazu
橋本 昌和
TAKEMOTO, Tatsuya
竹本 龍也
OTOI, Takeshige
音井 威重
TANIHARA, Fuminori
谷原 史倫
MORIIZUMI, Toshiyuki
森泉 俊幸
Title: METHOD FOR INTRODUCING Cas9 PROTEIN INTO FERTILIZED EGG OF MAMMAL
Abstract:
The present application provides a method for introducing the Cas9 protein into the fertilized egg of a mammal, and involves stages for (a) placing a mixture comprising a fertilized egg and a solution containing the Cas9 protein between electroporation electrodes and (b) applying a voltage between the electrodes. Further, using this method, it is possible to produce a fertilized egg of a mammal that contains the Cas9 protein, to edit the genome in a fertilized egg of a mammal, to create a genomically edited fertilized egg of a mammal, and to create a genetically modified animal.