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1. (WO2017168331) LINKED DUPLEX FRAGMENT SEQUENCING

Pub. No.:    WO/2017/168331    International Application No.:    PCT/IB2017/051778
Publication Date: Fri Oct 06 01:59:59 CEST 2017 International Filing Date: Wed Mar 29 01:59:59 CEST 2017
IPC: C12Q 1/68
C12N 15/10
C40B 30/04
C40B 40/06
Applicants: BOREAL GENOMICS, INC.
Inventors: MARZIALI, Andrea
PEL, Joel
Title: LINKED DUPLEX FRAGMENT SEQUENCING
Abstract:
The invention generally relates to sequencing library preparation methods. In certain embodiments, two or more template nucleic acids are joined together by a linking molecule, such as a PEG derivative. Identical copies of a nucleic acid fragment or both strands of a duplex fragment may be linked together. The linked nucleic acids are amplified, creating linked amplicons. Emulsion PCR with linked primers creates linked template nucleic acids for seeding sequencing clusters and errors can be readily identified by their presence on only one of the linked fragments.