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1. (WO2017156844) KIT FOR EVALUATING SPERM QUALITY AFTER IN VITRO CAPACITATION AND METHOD OF USE THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2017/156844 International Application No.: PCT/CN2016/081276
Publication Date: 21.09.2017 International Filing Date: 06.05.2016
IPC:
C12Q 1/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
Applicants:
四川大学华西第二医院 THE WEST CHINA SECOND UNIVERSITY HOSPITAL OF SICHUAN [CN/CN]; 中国四川省成都市 人民南路3段20号陈颖姣 CHEN Yingjiao No. 20, Section 3, Renmin South Road Chengdu, Sichuan 610041, CN
Inventors:
马芳 MA, Fang; CN
马黔红 MA, Qianhong; CN
潘倩 PAN, Qian; CN
马学 MA, Xue; CN
Agent:
成都天嘉专利事务所(普通合伙) CHENGDU TIANJIA PATENT OFFICE (GENERAL PARTNERSHIP); 中国四川省成都市 武侯区高攀路2号魏玛国际5栋20层20-21室苏丹 Room 2020、2021,20th Floor, No.5 Building of Weimar International (SU,Dan) No.2 Gaopan Road, Wuhou District Chengdu, Sichuan 610041, CN
Priority Data:
201610147270.416.03.2016CN
Title (EN) KIT FOR EVALUATING SPERM QUALITY AFTER IN VITRO CAPACITATION AND METHOD OF USE THEREOF
(FR) KIT D'ÉVALUATION DE LA QUALITÉ DU SPERME APRÈS CAPACITATION IN VITRO ET SON PROCÉDÉ D'UTILISATION
(ZH) 一种体外获能后精子质量评估的试剂盒及其使用方法
Abstract:
(EN) Provided are a kit for evaluating the sperm quality after in vitro capacitation and a method of use thereof. The kit comprises a detection buffer 1, a BWW culture solution, a solution for in vitro capacitation, a fluorescent reagent, a sialidase standard stock solution and a detection buffer 2. The detection buffer 1 is a phosphate buffer or a BWW culture solution. The detection buffer 2 is a phosphate buffer containing 0.05 mmol/l of sodium acetate and 0.25 μg/μl of bovine serum albumin.
(FR) L'invention concerne un kit pour évaluer la qualité du sperme après la capacitation in vitro et un procédé d'utilisation de celui-ci. Le kit comprend un tampon de détection 1, un milieu de culture Biggers-Whitten-Whittingham (BWW), une solution de capacitation in vitro, un réactif fluorescent, une solution mère standard de sialidase et un tampon de détection 2. Le tampon de détection 1 est un tampon phosphate ou un milieu de culture BWW. Le tampon de détection 2 est un tampon phosphate contenant 0,05 mmol/l d'acétate de sodium et 0,25 μg/μl d'albumine de sérum bovin.
(ZH) 本发明提供了一种体外获能后精子质量评估的试剂盒及其使用方法。所述的试剂盒包括检测缓冲液1、BWW培养液、体外获能液、荧光试剂、唾液酸酶标准原液和检测缓冲液2;所述的检测缓冲液1为磷酸缓冲液或者BWW培养液;所述的检测缓冲液2为含0.05 mmol/l 乙酸钠和0.25μg/μl牛血清白蛋白的磷酸缓冲液。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)