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1. (WO2017156843) KIT FOR EVALUATING SPERM QUALITY AND METHOD OF USE THEREOF

Pub. No.:    WO/2017/156843    International Application No.:    PCT/CN2016/081268
Publication Date: Fri Sep 22 01:59:59 CEST 2017 International Filing Date: Sat May 07 01:59:59 CEST 2016
IPC: G01N 33/68
Applicants: THE WEST CHINA SECOND UNIVERSITY HOSPITAL OF SICHUAN
四川大学华西第二医院
Inventors: MA, Fang
马芳
PAN, Qian
潘倩
XU, Chen
徐晨
Title: KIT FOR EVALUATING SPERM QUALITY AND METHOD OF USE THEREOF
Abstract:
Provided are a kit for evaluating sperm quality and a method of use thereof. The kit comprises a cell lysis buffer without an enzyme activity inhibitor, a detection buffer 1, a protease inhibitor cocktail, a reagent A solution, a reagent B solution, bovine serum albumin, a fluorescent reagent, a sialidase standard stock solution and a detection buffer 2. The detection buffer 1 is a phosphate buffer or a BWW culture solution. The reagent A solution comprises 1% BCA disodium salt, 2% anhydrous sodium carbonate, 0.16% sodium tartrate, 0.4% sodium hydroxide, and 0.95% sodium bicarbonate; and the ingredients mentioned above are mixed and the pH value is adjusted to 11.2-11.3. The reagent B solution is 4% copper sulfate. The detection buffer 2 is a phosphate buffer containing 0.05 mmol/l of sodium acetate and 0.25 μg/μl of bovine serum albumin.