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1. (WO2017156634) DEVICES AND METHODS FOR CELLULAR SECRETION ANALYSIS
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Pub. No.: WO/2017/156634 International Application No.: PCT/CA2017/050341
Publication Date: 21.09.2017 International Filing Date: 17.03.2017
IPC:
C12Q 1/02 (2006.01) ,B81B 1/00 (2006.01) ,C12M 1/34 (2006.01) ,C12Q 1/00 (2006.01) ,G01N 33/483 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/52 (2006.01) ,G01N 35/00 (2006.01) ,G01N 35/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
B PERFORMING OPERATIONS; TRANSPORTING
81
MICRO-STRUCTURAL TECHNOLOGY
B
MICRO-STRUCTURAL DEVICES OR SYSTEMS, e.g. MICRO-MECHANICAL DEVICES
1
Devices without movable or flexible elements, e.g. micro-capillary devices
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52
Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
10
Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Applicants:
THE UNIVERSITY OF BRITISH COLUMBIA [CA/CA]; c/o University - Industry Liaison Office #103 - 6190 Agronomy Road Vancouver, British Columbia V6T 1Z3, CA
Inventors:
HANSEN, Carl Lars Genghis; CA
LISAINGO, Kathleen; CA
LECAULT, Veronique; CA
Agent:
MACINS, Andris; CA
Priority Data:
62/309,66317.03.2016US
Title (EN) DEVICES AND METHODS FOR CELLULAR SECRETION ANALYSIS
(FR) DISPOSITIFS ET PROCÉDÉS D'ANALYSE DE SÉCRÉTION CELLULAIRE
Abstract:
(EN) Methods and devices for identifying a cell population comprising an effector cell exhibiting an extracellular effect are provided. The method comprises retaining in a plurality of open chambers a plurality of cell populations, each optionally comprising one or more effector cells. The open chambers can each comprise a readout particle population, and the open chambers are present in a first component of a device comprising a first component and optionally a second component. The open chambers have an average aspect ratio of ≥ 0.6 and the first component forms a reversible seal with the second component. The method further comprises incubating the plurality of cell populations or a subset thereof, and the one or more readout particles, or a subset thereof, within the chambers, assaying the cell populations for the presence of the extracellular effect, wherein the readout particle(s) provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more cells within one or more cell populations of the plurality exhibits the extracellular effect.
(FR) La présente invention décrit des procédés et des dispositifs d'identification d'une population de cellules comprenant une cellule effectrice faisant preuve d'un effet extracellulaire. Le procédé comprend la rétention dans une pluralité de chambres ouvertes d'une pluralité de populations de cellules, comprenant chacune éventuellement une ou plusieurs cellules effectrices. Les chambres ouvertes peuvent chacune comprendre une population de particules de lecture, et les chambres ouvertes sont présentes dans un premier composant d'un dispositif comprenant un premier composant et éventuellement un second composant. Les chambres ouvertes présentent un rapport d'aspect moyen ≥ 0,6 et le premier composant forme un joint réversible avec le second composant. Le procédé comprend en outre l'incubation de la pluralité de populations de cellules ou d'un sous-ensemble de ces dernières, et desdites particules de lecture, ou d'un sous-ensemble de ces dernières, à l'intérieur des chambres, analysant les populations de cellules en vue de détecter la présence de l'effet extracellulaire, lesdites particules de lecture fournissent une lecture directe ou indirecte de l'effet extracellulaire, et la détermination sur la base des résultats de l'étape d'essai, du fait qu'une ou plusieurs cellules à l'intérieur d'une ou plusieurs populations de cellules de la pluralité font preuve de l'effet extracellulaire.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)