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1. (WO2017156580) COLONY FORMING MEDIUM AND USE THEREOF

Pub. No.:    WO/2017/156580    International Application No.:    PCT/AU2017/050228
Publication Date: Fri Sep 22 01:59:59 CEST 2017 International Filing Date: Wed Mar 15 00:59:59 CET 2017
IPC: C12N 5/0775
A61K 35/28
Applicants: CYNATA THERAPEUTICS LIMITED
Inventors: SLUKVIN, Igor
UENISHI, Gene
HEI, Derek
DRIER, Diana
Title: COLONY FORMING MEDIUM AND USE THEREOF
Abstract:
The invention relates to a method for producing a mesenchymal stem cell (MSC), the method comprising culturing a primitive mesoderm cell in a mesenchymal colony forming medium (M-CFM) comprising LiCl and FGF2, but excluding PDGF, under normoxic conditions for sufficient time for a mesenchymal colony to form, and culturing the mesenchymal colony adherently to produce the MSC, wherein the MSC has superior T-cell immunosuppressive properties relative to an MSC not produced in said M-CFM. The invention also relates to an MSC produced by the method, a population of MSCs produced by the method, a therapeutic composition comprising the MSC produced by the method, an M-CFM and an M-CFM in concentrated form, and method and uses of the MSC or population in treating a disease.