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1. (WO2017156356) METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES

Pub. No.:    WO/2017/156356    International Application No.:    PCT/US2017/021715
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Sat Mar 11 00:59:59 CET 2017
IPC: C12N 15/63
C12N 1/21
A61K 38/00
C12N 15/11
C12N 15/62
C12N 15/31
C12N 15/77
C12P 21/00
C12R 1/16
C12R 1/19
Applicants: THE JOHNS HOPKINS UNIVERSITY
TRUSTEES OF BOSTON UNIVERSITY
Inventors: BISHAI, William R.
MURPHY, John R.
CHEUNG, Laurene
GUPTA, Shashank
BULLEN, Cynthia K.
Title: METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES
Abstract:
The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.