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1. (WO2017156264) NUCLEIC ACID NANOSWITCH CATENANES

Pub. No.:    WO/2017/156264    International Application No.:    PCT/US2017/021562
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Fri Mar 10 00:59:59 CET 2017
IPC: B82Y 15/00
B82Y 5/00
C12P 19/34
C12Q 1/68
Applicants: CHILDREN'S MEDICAL CENTER CORPORATION
DANA-FARBER CANCER INSTITUTE, INC.
Inventors: WONG, Wesley, Philip
SHIH, William, M.
Title: NUCLEIC ACID NANOSWITCH CATENANES
Abstract:
The present disclosure provides nucleic acid-based nanoswitch catenanes and methods of use. A nanoswitch catenane may include a single-stranded nucleic acid comprising a first and second terminal domain linked to each other to form a host ring by one of a first, second or third switchable bridges, wherein the first switchable bridge is formed in the presence of a reaction agent through the reaction of two cognate functional groups, each linked to a terminal domain of the single-stranded nucleic acid, wherein the second switchable bridge is formed in the presence of a biomolecule of interest through binding of the biomolecule of interest to two cognate antibodies, each linked to a terminal domain of the single stranded nucleic acid, and wherein the third switchable bridge is a link between two cognate functional groups that breaks in the presence of a dissociation agent. A nanoswitch catenane may also include a circular nucleic acid guest ring catenated with the host ring.