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1. (WO2017156126) LIGHT-MEDIATED POLYMERASE CHAIN REACTION AMPLIFICATION AND PRODUCT DETECTION SYSTEM AND METHODS OF USE

Pub. No.:    WO/2017/156126    International Application No.:    PCT/US2017/021359
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Thu Mar 09 00:59:59 CET 2017
IPC: B01L 3/00
B01L 7/00
G01N 21/64
G01N 35/02
Applicants: PIONEER HI-BRED INTERNATIONAL, INC.
Inventors: BIRO, Ronald L
HUNT, Beau
Title: LIGHT-MEDIATED POLYMERASE CHAIN REACTION AMPLIFICATION AND PRODUCT DETECTION SYSTEM AND METHODS OF USE
Abstract:
A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection subsystem. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.