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1. (WO2017155869) COMPOSITIONS AND METHODS FOR IDENTIFYING RARE CELLS

Pub. No.:    WO/2017/155869    International Application No.:    PCT/US2017/020905
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Tue Mar 07 00:59:59 CET 2017
IPC: C01F 7/04
C01G 37/10
C11D 3/12
C11D 9/22
C12N 11/08
C12Q 1/68
G01N 21/75
Applicants: X-ZELL INC.
Inventors: BHAKDI, Sebastian, Chakrit, Punyaratabandhu
Title: COMPOSITIONS AND METHODS FOR IDENTIFYING RARE CELLS
Abstract:
Disclosed herein are compositions and methods of fixing and staining rare cells. Farther, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identity a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensify; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such, that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a predetermined threshold, classify the cell of interest as a CTC.