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1. (WO2017155858) NUCLEIC ACID SEQUENCE IDENTIFICATION USING SOLID-PHASE CYCLIC SINGLE BASE EXTENSION

Pub. No.:    WO/2017/155858    International Application No.:    PCT/US2017/020887
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Tue Mar 07 00:59:59 CET 2017
IPC: C12Q 1/68
G01N 21/00
G01N 21/76
G01N 33/53
Applicants: INSILIXA, INC.
Inventors: HASSIBI, Arjang
Title: NUCLEIC ACID SEQUENCE IDENTIFICATION USING SOLID-PHASE CYCLIC SINGLE BASE EXTENSION
Abstract:
The present disclosure provides methods, devices and systems for detecting a presence of a nucleic acid molecule having a nucleic acid sequence. Detection of cyclic single base extension can be used to detect a nucleic acid molecule hybridized to a probe and detect a presence of a nucleic acid. The methods disclosed herein can detect a nucleic acid molecule present in a nucleic acid sample at low concentrations and in the presence of background nucleic acids having high sequence similarity.