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1. (WO2017155408) IMPROVED CRISPR-CAS9 GENOME EDITING TOOL

Pub. No.:    WO/2017/155408    International Application No.:    PCT/NL2017/050154
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Sat Mar 11 00:59:59 CET 2017
IPC: C12N 15/11
C12N 15/09
C07K 14/205
Applicants: ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM
WAGENINGEN UNIVERSITEIT
Inventors: LOUWEN, Rogier Petrus Leonardus
VAN DER OOST, John
VAN BAARLEN, Peter
Title: IMPROVED CRISPR-CAS9 GENOME EDITING TOOL
Abstract:
The invention relates to a Cas-based, preferably Cas9-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cas9 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.