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1. (WO2017155055) METHOD FOR MANUFACTURING CULTURE PRODUCT LIQUID

Pub. No.:    WO/2017/155055    International Application No.:    PCT/JP2017/009546
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Fri Mar 10 00:59:59 CET 2017
IPC: C12N 5/071
C12N 5/0775
C12M 1/00
Applicants: KintaroCellsPower Co., Ltd.
株式会社金太郎CellsPower
Inventors: GLADKOV Alexei
グラドコフ アレクセイ
Title: METHOD FOR MANUFACTURING CULTURE PRODUCT LIQUID
Abstract:
[Problem] To provide a method for manufacturing a culture product liquid, whereby a culture product liquid is manufactured which includes a predetermined metabolite secreted from a single type of stem cell. [Solution] The method for manufacturing a culture product liquid according to the present invention comprises: extracting an intermediate-layer bone marrow liquid positioned in an intermediate layer from bone marrow liquid separated in layered fashion and culturing the intermediate-layer bone marrow liquid together with a culture liquid; fixing first stem cells to a bottom surface of a first culture container and collecting the first stem cells from the first culture container when the total area of the first stem cells reaches a first target ratio of the bottom-surface area of the first culture container; culturing second stem cells together with the culture liquid, the second stem cells being positioned in a bottom layer from among the first stem cells separated in layered fashion, while collecting the second stem cells; and fixing the second stem cells to the bottom surface of a second culture container, and extracting a culture product liquid including a predetermined metabolite secreted from a single type of second stem cells grown from the second culture container when the total area of the second stem cells reaches a second target ratio of the bottom-surface area of the second culture container.