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1. (WO2017154880) CELL CULTURE DEVICE AND CELL CULTURE METHOD

Pub. No.:    WO/2017/154880    International Application No.:    PCT/JP2017/008947
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Wed Mar 08 00:59:59 CET 2017
IPC: C12M 1/00
C12M 1/04
C12M 3/00
C12N 5/07
Applicants: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
国立研究開発法人産業技術総合研究所
Inventors: SUGIURA Shinji
杉浦 慎治
SATOH Taku
佐藤 琢
KANAMORI Toshiyuki
金森 敏幸
SHIN Kazumi
進 和美
Title: CELL CULTURE DEVICE AND CELL CULTURE METHOD
Abstract:
This cell culture device is provided with a storage tank having one or more cell culture units. The cell culture units each have: a main culture medium chamber that has a circulation space in which a culture medium for cells circulates; a permeable diaphragm of which one surface, to which the cells can adhere, faces the circulation space; a first culture medium storage chamber that has an airtight structure, and in which the culture medium is stored; a second culture medium storage chamber in which the culture medium is stored; a culture medium lead-in flow path that leads the culture medium from the first culture medium storage chamber to the circulation space of the main culture medium chamber; and a culture medium lead-out flow path that leads the culture medium from the circulation space to the second culture medium storage chamber. The storage tank has a vent hole that supplies and discharges a gas to and from the first culture medium storage chamber.