Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2017154869) METHOD FOR PRODUCING MUTANT HUMAN ERYTHROPOIETIN
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/154869 International Application No.: PCT/JP2017/008907
Publication Date: 14.09.2017 International Filing Date: 07.03.2017
IPC:
C12P 21/02 (2006.01) ,C07K 1/16 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
16
by chromatography
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
JCRファーマ株式会社 JCR PHARMACEUTICALS CO., LTD. [JP/JP]; 兵庫県芦屋市春日町三番十九号 3-19, Kasuga-cho, Ashiya-shi, Hyogo 6590021, JP
キッセイ薬品工業株式会社 KISSEI PHARMACEUTICAL CO., LTD. [JP/JP]; 長野県松本市芳野19番48号 19-48, Yoshino, Matsumoto-shi, Nagano 3998710, JP
Inventors:
柿本 真司 KAKIMOTO, Shinji; JP
マテヴ ミロスラヴ MATEV, Miroslav; JP
福井 剛 FUKUI, Tsuyoshi; JP
秦野 勇吉 HATANO, Yukichi; JP
谷 純也 TANI, Junya; JP
三原 和敏 MIHARA, Kazutoshi; JP
高橋 健一 TAKAHASHI, Kenichi; JP
杉村 厚 SUGIMURA, Atsushi; JP
Agent:
早坂 巧 HAYASAKA, Takumi; JP
Priority Data:
2016-04533809.03.2016JP
Title (EN) METHOD FOR PRODUCING MUTANT HUMAN ERYTHROPOIETIN
(FR) PROCÉDÉ DE PRODUCTION D’ÉRYTHROPOÏÉTINE HUMAINE MUTANTE
(JA) 変異型ヒトエリスロポエチンの製造方法
Abstract:
(EN) Disclosed is a method for producing mutant human erythropoietin with high purity and high yield. The method is a method for producing mutant human erythropoietin, comprising causing the mutant human erythropoietin to be produced by a transgenic mammalian animal cell and then subjecting a culture supernatant to hydrophobic column chromatography, multimodal anion-exchange column chromatography, anion-exchange column chromatography, phosphate-affinity column chromatography and gel filtration column chromatography sequentially in this order.
(FR) La présente invention décrit un procédé de production d’érythropoïétine humaine mutante sous une pureté élevée et un rendement élevé. Le procédé est un procédé de production d’érythropoïétine humaine mutante, comprenant le fait d’entraîner la production d’érythropoïétine humaine mutante par une cellule animale de mammifère transgénique et ensuite la soumission d’un surnageant de culture à la chromatographie sur colonne hydrophobe, la chromatographie multimodale sur colonne d’échange d’anions, la chromatographie sur colonne à échange d’anions, la chromatographie sur colonne par affinité phosphate et la chromatographie sur colonne à filtration sur gel séquentiellement dans cet ordre.
(JA) 高純度かつ高収率の変異型ヒトエリスロポエチンの製造方法が開示されている。当該方法は,形質転換哺乳動物細胞に変異型ヒトエリスロポエチンを産生させ,培養上清を疎水性カラムクロマトグラフィー,マルチモーダル陰イオン交換カラムクロマトグラフィー,陰イオン交換カラムクロマトグラフィー,リン酸基親和性カラムクロマトグラフィー,ゲルろ過カラムクロマトグラフィーにこの順で付すことによる,変異型ヒトエリスロポエチンの製造方法である。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)