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1. (WO2017154013) AN ASSAY AND KIT FOR DETECTION OF ENDOTOXIN

Pub. No.:    WO/2017/154013    International Application No.:    PCT/IN2016/050397
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Sat Nov 12 00:59:59 CET 2016
IPC: G01N 33/00
Applicants: NANODX HEALTHCARE PVT. LTD.
Inventors: GUPTA, Shalini
SRITHARAN, Venkataraman
KALITA, Prasanta
Title: AN ASSAY AND KIT FOR DETECTION OF ENDOTOXIN
Abstract:
The present invention relates to a membrane based assay method, device and kit for rapid detection and/or quantification of endotoxins in aqueous solutions and test samples. The kit as per the present invention comprises lipopolysaccharide (LPS) affinity ligand conjugated with gold nanoparticles (GNPs); a membrane device comprising an endotoxin affinity membrane positioned parallelly to one or more layer(s) of a hydrophilic material, which are optionally secured in an enclosure; and optionally comprising an indicator chart for quantification of endotoxins in the sample. The method comprises placing the sample suspected of endotoxin contamination on a surface of a membrane comprised in a membrane device; placing once or more a suspension of LPS-affinity ligand conjugated with GNPs over the same area as the sample placed and detecting the presence of endotoxin if the colour signal appears and based on its intensity quantifying the endotoxin levels.