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1. (WO2017153802) METHOD OF DIFFERENTIATING PLURIPOTENT STEM CELLS

Pub. No.:    WO/2017/153802    International Application No.:    PCT/IB2016/000807
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Tue Mar 08 00:59:59 CET 2016
IPC: C12N 5/071
C12N 5/0735
C12N 5/074
Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
THE FOUNDATION FOR THE PROMOTION OF INDUSTRIAL SCIENCE
Inventors: LECLERC, Eric
SAKAI, Yasuyuki
KIMURA, Keiichi
MIYAJIMA, Atsushi
KIDO, Taketomo
Title: METHOD OF DIFFERENTIATING PLURIPOTENT STEM CELLS
Abstract:
An object is to provide a method of differentiating pluripotent stem cells. A method of differentiating pluripotent stem cells, the method comprising steps of: seeding the pluripotent stem cells in a container provided with seeding medium! differentiating the pluripotent stem cells in the container; transferring the pluripotent stem cells from the container into a chamber of a bioreactor when the pluripotent stem cells reach their progenitor stage; and maturing the pluripotent stem cells in the chamber, wherein a floor of the chamber includes a concave and a convex, fluid of medium flows in the chamber and oxygen is supplied into the chamber.