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1. (WO2017153766) EPIGENETICALLY STABLE CLONED PLANTS

Pub. No.:    WO/2017/153766    International Application No.:    PCT/GB2017/050640
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Sat Mar 11 00:59:59 CET 2017
IPC: C12N 15/82
Applicants: UNIVERSITY OF WARWICK
Inventors: GUTIERREZ-MARCOS, Jose
WIBOWO, Anjar
Title: EPIGENETICALLY STABLE CLONED PLANTS
Abstract:
A method of cloning plants involves a reprogramming of somatic plant cells of a selected parent plant into undifferentiated initials by expressing RKD4, BBM, LEC2 or FUS3 transcription factors using a two-component inducible expression system. New plants are then recovered by growing the transformed cells in the absence of the inducer. Regenerated plants are fertile and retain epigenetic characteristics of the somatic cells prior to the reprogramming. No changes in gene sequence take place. Regenerated plants are selected so they have at least one epigenetic characteristic of the parent plant. This approach allows a plant, for example Elaeis guineensis or Phalaenopsis sp. with desirable epigenetic character to be cloned.