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1. (WO2017153266) PROCESS FOR RNA PRODUCTION

Pub. No.:    WO/2017/153266    International Application No.:    PCT/EP2017/054989
Publication Date: Fri Sep 15 01:59:59 CEST 2017 International Filing Date: Sat Mar 04 00:59:59 CET 2017
IPC: C12P 19/30
C12N 15/10
Applicants: PROSOL S.P.A.
Inventors: BONVICINI, Daniele
LISSONI, Stefano
TOGNI, Ermanno
Title: PROCESS FOR RNA PRODUCTION
Abstract:
Process for the production of RNA from microbial cells,which comprises steps of treating such cells to release the cell content thereof, including RNA; separating the RNA from other soluble cellular compounds via an osmotic process by the use of semi-permeable membranes and concentration, obtaining a retentate enriched in RNA; adjusting the retentate pH to a value between 2 and 3, thus causing the precipitation of RNA, and recovering the precipitated RNA by filtration or centrifugation, wherein the aforesaid osmotic process is a diafiltration and prior to adjusting the retentate pH to a value between 2 and 3, a salting out step is performed at a pH between 3.5 and 5.0 with ensuing precipitation of the proteins that are present in the retentate.